The reaction volume of a traditional PCR machine is usually more than 20 μL. The larger the sample volume, the more detection reagents are needed, and the corresponding detection cost is higher. Furthermore, traditional products have a long reaction time, requiring at least 1-2 hours, which cannot achieve rapid on-site detection. In addition, traditional products are not only expensive, but also bulky and poor in portability. In comparison, the microfluidic chip PCR instrument has obvious advantages of portability, short detection time, simple operation, and low detection cost.